qPCR Master Mix Beads

Lyophilization DryerWe developed a set of blank lyophilized qPCR master mix reagent beads that are based on carefully selected, robust set of lyo-friendly enzymes, master mixes, excipients, and additives. Blank qPCR master mix beads have all the assay components for a reaction except the primers, probe and template.

Developing stable, sensitive, and reproducible molecular diagnostic (PCR) assays is usually a challenging process especially for point-of-care (POC) applications. Researchers frequently develop assays in liquid format first and delay reagent formula drying stabilizing efforts until later date. In many cases, this leads to enzyme re-selection and complete assay re-development, loss of valuable time, and resource shortages.

To resolve many of the assay design problems, we developed a set of blank lyophilized qPCR master mix reagent beads.

Using our blank master mix beads from the start of your assay development process has several benefits, including the following:

  • Reducing time and cost:
    • The time-consuming and costly steps of selecting enzymes and excipients are eliminated
    • The process development and validation times are significantly reduced
  • Reducing Risk:
    • The risks of failure in assay sensitivity and stability are dramatically reduced

Blank master mix beads have all the assay components for a reaction except the primers, probes, and template. We can accommodate clients requiring blank beads with enzymes from a specific source. Our beads are customizable to fit specific assay needs.

A variety of packaging options are available: 8-strip PCR tubes, 96-well plates, or any other custom format. Our standard beads support 20 ┬ÁL reactions but can also be customized for specific assay volumes.

Blank qPCR Beads

Product No. Description Quantity Price
LL-QM-001 qPCR Master Mix beads for 20 uL reactions packaged in PCR 8-strips 1 x 8 strips Call for Pricing
LL-QL-001 qPCR Master Mix beads for 20 uL reactions packaged in PCR 8-strips 1 x 8 strips Call for Pricing
LL-QE-001 qPCR Master Mix beads for 20 uL reactions packaged in PCR 8-strips 1 x 8 strips Call for Pricing
LL-QR-001 qPCR Master Mix beads for 20 uL reactions packaged in PCR 8-strips 1 x 8 strips Call for Pricing
LL-QT-001 Selection test kit with 4 different qPCR Master Mix beads for 20 uL reactions, each Master Mix packaged in separate PCR 8-strip 4 x 8 strips Call for Pricing
LL-QM-002 qPCR Master Mix beads for 20 uL reactions packaged in 96 well plates 1 x 96 plate Call for Pricing
LL-QL-002 qPCR Master Mix beads for 20 uL reactions packaged in 96 well plates 1 x 96 plate Call for Pricing
LL-QE-002 qPCR Master Mix beads for 20 uL reactions packaged in 96 well plates 1 x 96 plate Call for Pricing
LL-QR-002 qPCR Master Mix beads for 20 uL reactions packaged in 96 well plates 1 x 96 plate Call for Pricing

The use of lyophilized or freeze-dried reagents has become an POC industry standard as the best way to incorporate assay reagents into disposable cartridges

  • Dried reagent(s) have long shelf-life (stability)
  • Cold-chain storage and shipment is averted
  • A more cost-effective process than lyophilizing in cartridges
  • Beads can be tested before cartridge assembly, mitigating risk of wasting whole cartridge with multiplex reactions
  • Beads lyophilizing process takes 100 times less freeze-dryer shelf space than lyophilizing whole cartridge